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. 2020 Mar 10;15(3):e0230080. doi: 10.1371/journal.pone.0230080

Table 1. Primers used for real-time polymerase chain reaction.

Name Symbol Species Forward (5′ to 3′) Reverse (5′ to 3′) Size (bp)
AIF1 Aif1 mouse atcaacaagcaattcctcgatga cagcattcgcttcaaggacata 144
Arg1 Arg1 mouse ctccaagccaaagtccttagag aggagctgtcattagggacatc 185
CCL2 Ccl2 mouse ttaaaaacctggatcggaaccaa gcattagcttcagatttacgggt 121
CCL3 Ccl3 mouse ttctctgtaccatgacactctgc cgtggaatcttccggctgtag 100
CCL4 Ccl4 mouse ttcctgctgtttctcttacacct ctgtctgcctcttttggtcag 121
CCL5 Ccl5 mouse gctccaatcttgcagtcgtg gagcagctgagatgcccatt 242
CCL7 Ccl7 mouse gctgctttcagcatccaagtg ccagggacaccgactactg 135
CCR1 Ccr1 mouse tggtgggcaatgtcctagtg aagtaacagttcgggccctc 300
CCR2 Ccr2 mouse atccacggcatactatcaacatc caaggctcaccatcatcgtag 104
CCR3 Ccr3 mouse accccgtacaacctggttct ccaacaaaggcgtagattactgg 158
CCR5 Ccr5 mouse ttttcaagggtcagttccgac ggaagaccatcatgttacccac 158
CRMP2 Dpysl2 mouse tcaaaggtggcaagattgtgaa ggaatcaccattctggagtgg 145
CXCL1 Cxcl1 mouse ctgggattcacctcaagaacatc cagggtcaaggcaagcctc 117
CXCL2 Cxcl2 mouse tccagagcttgagtgtgacg tggttcttccgttgagggac 201
CXCR2 Cxcr2 mouse gggtggggagttcgtgtaga aggtgctaggatttgagcct 200
ELANE Elane mouse caggaacttcgtcatgtcagc agcagttgtgatgggtcaaag 162
ENO2 Eno2 mouse tgagaataaatccttggagctggt ggtcatcgcccactatctgg 302
GAP43 Gap43 mouse tctgctactaccgatgcagc tggaggacggggagttatcag 181
GAPDH Gapdh mouse gctacactgaggaccaggttgt ctcctgttattatgggggtctg 306
GAPDH Gapdh human ggagcgagatccctccaaaat ggctgttgtcatacttctcatgg 197
GAPDH* Gapdh mouse aggtcggtgtgaacggatttg tgtagaccatgtagttgaggtca 123
IL-1β Il1b mouse gcaactgttcctgaactcaact atcttttggggtccgtcaact 89
IL-4 Il4 mouse tctcgaatgtaccaggagccatatc agcaccttggaagccctacaga 183
MCPIP Zc3h12a mouse acgaagcctgtccaagaatcc taggggcctctttagccaca 115
MPO Mpo mouse agggccgctgattatctacat ctcacgtcctgataggcaca 152
Ym-1 Chil3 mouse ggtggacacagaatggttcttc ccaggagcgttagtgacagc 128

AIF: allograft inflammatory factor, Arg: arginase, CCL: chemokine (C-C motif) ligand, CCR: chemokine (C-C motif) receptor, Chil: chitinase-like, CRMP: collapsin response mediator protein, CXCL: chemokine (C-X-C motif) ligand, CXCR: chemokine (C-X-C motif) receptor, ELANE: neutrophil elastase, Eno: neuron-specific enolase, GAP: growth associated protein, GAPDH: glyceraldehyde-3-phosphate dehydrogenase, GFAP: glial fibrillary acidic protein, IL: interleukin, MCPIP: monocyte chemotactic protein-1 induced protein, MPO: myeloperoxidase, * = not species specific.