Fig 2. Chiron concentration and seeding density uniquely affect cardiac differentiation outcome.

DOE (design of experiments) surface plots of directed differentiation outcomes in human induced pluripotent stem cell lines obtained from commercial (GiPSC; A), national bank (NCRM-5; B), and research institution (WTC11; C) sources. Red and pink circles indicate design points above and below predicted values, respectively. N = 9 per group per condition from 3 biological replicates (i.e., batches of hiPSC-cardiomyocytes).