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. 2020 Mar 10;15(3):e0230001. doi: 10.1371/journal.pone.0230001

Fig 4. HiPSC-cardiomyocytes increase oxidative phosphorylation with lactate purification.

Fig 4

(A) Cardiomyocytes were differentiated from Gibco hiPSCs (GiPSCs). (B) Control cells (blue, 51% cTnT+) were cultured for the same duration (with no starvation) and fed glucose medium, and lactate purified cells (black, 82% cTnT+) underwent glucose starvation and lactate purification from day 13 to 21 of culture. (C-E) Oxygen consumption rate (OCR; C & D) and extracellular acidification rate (ECAR), were measured at basal metabolic levels using a Seahorse XFe96 Analyzer, and values were normalized using a LIVE/DEAD stain. OCR was calculated independent of cardiac purity (C, **P<0.01) and normalized by cardiac purity (D, P = 0.46). (E) The ratio OCR/ECAR of Control (unpurified, 51% cTnT+) and Lactate (lactate purified, 82% cTnT+) samples was calculated (*P<0.05). N = 10 per group, bars represent mean ± SEM. (RI: rock inhibitor, CH: chiron, IWP2: inhibitor of wnt protein 2, CDM3: cardiac differentiation media 3, RPMI/B27 +I: RPMI/B27 with insulin, LPM: lactate purification media, cTnT: cardiac troponin T.).