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. 2020 Feb 27;18(2):e3000639. doi: 10.1371/journal.pbio.3000639

Fig 11. Amygdalar pathways in the MDmc form synaptic triads.

Fig 11

(A–C) Labeled amygdalar terminals (black label with blue overlay) were large and contained round vesicles, RLs. Amygdalar terminals were also attached to EDs (green) through many puncta adherentia (A–C, arrowheads), which together covered a significant part of the dendritic surface. Synapses of amygdalar terminals with EDs were found mostly on thorns (A–C, arrowhead with asterisk on green shading). EDs innervated by amygdalar terminals also received synapses (A, arrow) from elements of inhibitory neurons (A, orange); amygdalar terminals also formed synapses with IDs (B, arrowhead with asterisk on orange shading). (D and E) Amygdalar terminals formed classical triadic arrangements with one ED (green) and one ID (orange). (F, G) Classic triadic arrangements are found in the LGN. Amygdalar terminals in A–E were labeled with the neural tracer FE and visualized with DAB. Yellow asterisks show silver-enhanced gold labeling for PV, and red asterisks in A show TMB rod-like labeling for CB in postsynaptic dendrites. Numbers in B–G indicate distance in nanometers between sections photographed. For color code, see Fig 10. Calibration bar in G applies to B–G. CB, calbindin; DAB, diaminobenzidine; ED, excitatory dendrite; FE, Fluoro- emerald; ID, inhibitory dendrite; LGN, lateral geniculate nucleus; MDmc, mediodorsal thalamic nucleus, magnocellular part; PV, parvalbumin; TMB, tetramethylbenzidine.