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. 2020 Mar 6;13:1979–1991. doi: 10.2147/OTT.S239258

Figure 5.

Figure 5

LINC00460 increases AGR2 expression via sponging miR-342-3p in HCC cells. (A and B) qRT-PCR and Western blot analysis were performed to determine AGR2 expression in HepG2 and Huh7 cells transfected with NC mimics, miR-342-3p mimics, or miR-342-3p mimics + LINC00460. (C) Relative mRNA expression of miR-342-3p in HCC tissues and adjacent normal tissues was examined using qRT‐PCR. (D) Pearson correlation analysis between miR-342-3p and LINC00460 expressions in 50 pairs of HCC tissues. (E) CCK8 assay was used to measure the proliferation of HepG2 and Huh7 cells transfected with empty vector, pcDNA3.1-LINC00460, pcDNA3.1-LINC00460 + miR-342-3p mimic, or pcDNA3.1-LINC00460 + si-AGR2. (F and G) Transwell assays were used to determine the migration and invasion of HepG2 and Huh7 cells transfected with empty vector, pcDNA3.1-LINC00460, pcDNA3.1-LINC00460 + miR-342-3p mimic, or pcDNA3.1-LINC00460 + si-AGR2. Scale bar, 100 μm. Data are representative of three independent experiments and shown as mean ± s.d. **P < 0.01, ***P < 0.001.