Figure 1.
The effect of preexisting HMGB1 removal on the viability of cultured beta cells. 1 × 105 MIN6 cells or 300 IEQs of murine islets were incubated with the CCK8 reagent for 2 h in a 37°C, 5% CO2 incubator. (A) CCK8 assay results of cultured primary islets. Data are presented as mean ± SD. ****, P < .0001 as calculated by unpaired t-test. (B) CCK8 assay results of cultured MIN6 cells. Data are presented as mean ± SD. ****, P < .0001 as calculated by unpaired t-test. (C) Viability of MIN6 cells determined by Annexin V and PI staining. A representative scatter plot is shown. *, P < .05 as calculated by unpaired t-test. (D) Cell viability of MIN6 cells was also determined by TMRE staining. 5 × 105 MIN6 cells were trypsinized and stained with 150 nM of TMRE for 5 min at RT in the dark. Data are presented as mean ± SD. ***, P < .0001 as calculated by unpaired t-test.