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The effect of HMGB1-depletion from the FBS on pancreatic islet cell culture. The amount of cytokines within culture supernatants of primary islets (150 IEQs) incubated 48 h with or without preexisting HMGB1 removal, measured via CBA. Data are presented as mean ± SD. **, P < .01 as calculated by unpaired t-test.
