Figure 4.

The effects of rHMGB1 re-addition on beta cell viability and function. After the cells were cultured for 48 h with various amounts of rHMGB1 (10 ng/mL or 100 ng/mL) cells were incubated with the CCK8 reagent for 2 h in a 37°C, 5% CO2 incubator. (A) CCK8 assay results of cultured primary islets (300 IEQs). Data are presented as mean ± SD. **, P < .01; *, P < .05 as calculated by unpaired t-test. (B) CCK8 assay results of cultured MIN6 cells (1 x 10⁵). Data are presented as mean ± SD. *, P < .05 as calculated by unpaired t-test. (C) GSIS of pancreatic islet cells cultured in HMGB1-depleted FBS-supplemented media with or without the addition of rHMGB1 (10 ng/mL). The islets were equilibrated for 1 h in 2 mM glucose solution and then incubated sequentially for 1 h each in 2 mM and 20 mM glucose solutions. Data are presented as mean ± SD. *, P < .05 as calculated by unpaired t-test.