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. 2020 Mar 3;9:e53169. doi: 10.7554/eLife.53169

Key resources table.

Reagent type
(species) or resource
Designation Source or reference Identifiers Additional
information
Cell line (Homo-sapiens) HEK293-6E National Research Council of Canada 11565
Cell line (Homo-sapiens) Expi293F Thermo Fisher Scientific A14527
Cell line (Homo-sapiens) Hep3B2.1–7 ATCC HB-8064
Antibody Anti-Human IgG-HRP (Goat polyclonal) SouthernBiotech 2040–05 1:4000 dilution
Recombinant DNA reagent pTT5 mammalian expression vector (used to express IgGs and Fabs) National Research Council of Canada N/A
Commercial assay or kit 1-Step Ultra TMB-ELISA Substrate Solution Thermo Fisher Scientific 34028
Commercial assay or kit PEGRx HT Hampton Research HR2-086
Commercial assay or kit PEG/Ion HT Hampton Research HR2-139
Commercial assay or kit JCSG-plus HT-96 Molecular Dimensions MD1-40
Chemical compound, drug Kifunensine Sigma K1140
Software, algorithm Pymol Schrödinger, LLC RRID:SCR_000305
Software, algorithm Phenix (Adams et al., 2010) https://www.phenix-online.org
Software, algorithm Coot (Emsley and Cowtan, 2004) http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Software, algorithm PDBePISA (Krissinel and Henrick, 2007) http://www.ebi.ac.uk/pdbe/pisa/
Software, algorithm abYsis system http://www.bioinf.org.uk/abysis/
Other Superdex 200 Increase 10/300 GL GE Healthcare 17517501
Other HisTrap FF column GE Healthcare 17531901
Other HiTrap Protein A HP column GE Healthcare 17040301
Other HCV 1b09 strain E1E2 sequence GenBank KJ187984.1