Table 3. Fiber diffraction analysis of MTs in various nucleotide-bound states.
GDP | GDP-Tx | GDP-BeF3- | GTP-BeF3- | GDP- AlFx | GTP-AlFx | GMPCPP | GMPPCP | GMPCP | |
---|---|---|---|---|---|---|---|---|---|
radius (nm) | 11.42 ± 0.10 | 10.87 ± 0.10 | 11.21 ± 0.25 | 11.16 ± 0.10 | 11.25 ± 0.84 | 11.18 ± 0.12 | 11.63 ± 0.10 | 11.62 ± 0.59 | 11.75 ± 0.53 |
avg. PF number | 12.91 ± 0.10 | 12.37 ± 0.10 | 12.29 ± 0.20 | 12.23 ± 0.10 | 13.43 ± 1.12 | 13.35 ± 0.13 | 13.29 ± 0.08 | 13.03 ± 0.91 | 13.55 ± 0.45 |
inter-PF distances (nm) | 5.50 ± 0.03 | 5.45 ± 0.01 | 5.67 ± 0.09 | 5.67 ± 0.02 | 5.21 ± 0.46 | 5.22 ± 0.05 | 5.45 ± 0.03 | 5.55 ± 0.38 | 5.40 ± 0.02 |
avg. monomer length (nm) | 4.06 ± 0.01 | 4.18 ± 0.01 | 4.07 ± 0.01 | 4.07 ± 0.01 | 4.05 ± 0.05 | 4.05 ± 0.01 | 4.18 ± 0.01 | 4.06 ± 0.01 | 4.17 ± 0.01 |
1 nm band peak position (nm−1) | 6.19 ± 0.01 | 6.02 ± 0.01 | 6.17 ± 0.01 | 6.17 ± 0.01 | 6.20 ± 0.05 | 6.20 ± 0.01 | 6.02 ± 0.01 | 6.20 ± 0.01 | 6.03 ± 0.01 |
*Values are Avg ± StdErr.