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. 2020 Mar 10;9:e50155. doi: 10.7554/eLife.50155

Table 4. Comparison between experimental and theoretical PF skew angles.

GDP BeF3- GMPCPP
MT type 13_3 14_3 12_3 13_3 13_3 14_3
θexp +0.10 ± 0.21
(n = 15)
−0.62 ± 0.05
(n = 27)
+0.60 ± 0.05
(n = 52)
−0.27 ± 0.07
(n = 9)
+0.33 ± 0.11
(n = 12)
−0.51 ± 0.04
(n = 12)
θthe +0.05 −0.74 +0.61 −0.31 +0.37 −0.44

Theoretical PF skew angles (θthe) were calculated according to Equation 9, using a = 40.9 Å, r = 9.4 Å, and δx = 48.95 Å for GDP-MTs. For GMPCPP MTs, the monomer spacing a was increased to 42.1 Å, and for BeF3- the inter-PF subunit rise was increased to 9.7 Å.