(A) Schematic representation of PHF19L and PHF19S and their domains. (B) Western blot analysis showing expression of PHF19L, PHF19S, EZH2, and GAPDH in RWPE1, PC3, and DU145 cells. *, non-specific bands (C) Summary of the main interactors of PHF19L and PHF19S identified by mass spectrometry (MS). PC3 cells stably expressing FLAG-tagged PHF19L or PHF19S, or FLAG-tagged empty vector (control), were subjected to FLAG affinity purification followed by MS. The table displays the score and the peptide count from two independent experiments. (D,E) Endogenous co-immunoprecipitation (IP) of PHF19L with EZH2 or SUZ12 in control (shCTR) and PHF19L-depleted (shPHF19L#1 or shPHF19L#4) PC3 cells (D) or DU145 cells (E). IgG was used as a control. *, non-specific band.