Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Cell line (Vero) | Kidney (normal, epithelial, adult) | ATCC | CCL-81 | |
Cell line (Rousettus aegyptiacus) | Kidney (normal, epithelial, adult) | (Biesold et al., 2011; Kühl et al., 2011) | RoNi/7.1 | |
Cell line (Pteropus alecto) | Kidney (normal, epithelial, adult) | (Crameri et al., 2009) | PaKiT01 | |
Virus strain | Replication competent, recombinant vesicular stomatitis Indiana virus expressing eGFP | (Miller et al., 2012; Wong et al., 2010) | rVSV-G | |
Virus strain | Replication competent, recombinant vesicular stomatitis Indiana virus expressing eGFP and EBOV GP in place of VSV G | (Miller et al., 2012; Wong et al., 2010) | rVSV-EBOV | |
Virus strain | Replication competent, recombinant vesicular stomatitis Indiana virus expressing eGFP and MARV GP in place of VSV G | (Miller et al., 2012; Wong et al., 2010) | rVSV-MARV | |
Reagent | Hoechst 33342 Fluorescent Stain | ThermoFisher | cat #: 62249 | |
Reagent | L-Glutamine Solution | ThermoFisher | cat #: 25030081 | |
Reagent | Gibco HEPES | ThermoFisher | cat #: 15630080 | |
Reagent | iTaq Universal SYBR Green Supermix | BioRad | cat #: 1725120 | |
Commercial assay or kit | Quick RNA Mini Prep Kit | Zymo | cat #: R1054 |
|
Commercial assay or kit | Invitrogen Superscript III cDNA Synthesis Kit | ThermoFisher | cat #: 18080051 | |
Software | MatCont (version 2.2) | (Dhooge et al., 2008) | MatCont | |
R | R version 3.6.0 | (R Development Core Team, 2019) | R |
*Note that primers for R. aegyptiacus and P. alecto β-Actin, IFN-α, and IFN-β genes are listed in the Supplementary file 6.