Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | WT-1 | Uniprot IGLV2-14 | Uniprot: P01704 | |
| Gene (Homo sapiens) | Pat-1 | This paper | GenBank: MK962887 | |
| Strain, strain background (E. coli) | BL21-codon+ (DE3)-RIL | Stratagene | ||
| Strain, strain background (E. coli) | XL1blue | Stratagene | ||
| Biological sample (Homo sapiens) | abdominal fat tissue | University hospital Heidelberg | ||
| Recombinant DNA reagent | pet28b-WT-1 | invitrogen | Point mutants obtained by site directed mutagenesis using NEBasechanger | |
| Recombinant DNA reagent | pet28b-Pat-1 | invitrogen | Point mutants obtained by site directed mutagenesis using NEBasechanger | |
| Recombinant protein | Pat-1 LC, Pat-1 VL, WT-1 LC, WT-1 VL, Pat-1 VL and WT-1 VLpoint mutants | This paper | Expression plasmids obtained from invitrogen (see: recombinant DNA reagent). Point mutant expression plasmids created using site directed mutagenesis (see: other). Protein purification according to Material and methods section | |
| Commercial assay or kit | Wizard Plus SV Mini-Prep DNA purification | Promega | A1460 | |
| Software, algorithm | Origin 2018b | OriginLab Corporation | ||
| Software, algorithm | SedFit | Peter Schuck | ||
| Software, algorithm | Discovery Studio | BIOVA | ||
| Software, algorithm | Pymol | Schrödinger, DeLano Scientific LLC | ||
| Software, algorithm | Adobe Illustrator | Adobe Inc | ||
| Software, algorithm | PLGS and DynamX | Waters.com | ||
| Other | NEBaseChanger | NEB | https://nebasechanger.neb.com | Primer design for site directed mutagenesis |