Figure 8. Ablation of neither TbPam18 nor TbPam16 does affect the formation of the presequence intermediate.

Left: Schematic representation of the stalled presequence intermediate induced by in vivo expression of the LDH-DHFR fusion protein in presence of aminopterin (AMT). Right: BN-PAGE analysis of the presequence intermediate in cell lines expressing myc-tagged LDH-DHFR in the background of RNAi against either TbPam18 or TbPam16 as indicated. Experiments were done exactly as described in Figure 7A.