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. 2020 Mar 2;9:e51102. doi: 10.7554/eLife.51102

Figure 6. The LXA4 pathway regulates effector function and metabolism of CD4+ T cells.

Figure 6.

Inguinal lymph nodes and spleens were harvested from EAU-challenged WT and Alox5-/- mice on day 13 post-immunization. (A) CD4+ T cells isolated from immunized WT and Alox5-/- mice were stimulated with anti-CD3 and anti-CD28 antibodies for 18 hr then subjected to glycolytic rate assay by Seahorse analyzer, n = 12 mice per group. (B–C) Bar graphs of (B) basal glycolysis and (C) compensatory glycolysis from the glycolytic rate assay in (A). (D) WT CD4+ T cells isolated from immunized WT and Alox5-/- mice were pretreated with vehicle or LXA4 in vitro and stimulated with anti-CD3 and anti-CD28 antibodies for 18 hr then subjected to glycolytic rate assay by Seahorse analyzer, n = 11 mice per group. (E–F) Bar graphs of (E) basal glycolysis and (F) compensatory glycolysis from the glycolytic rate assay in (D). *p<0.05, ***p<0.001, Wilcoxon matched-pairs signed rank test and Mann-Whitney test.