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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Bone Miner Res. 2019 Dec 12;35(3):550–570. doi: 10.1002/jbmr.3908

Figure 3: MCT4 Expression is Important for Maintenance of NP Cell Phenotype and Extracellular Matrix Composition.

Figure 3:

Representative immunostaining showing expression of important proteins in the intervertebral disc in 14 mo WT and MCT4 KO mice. A, A’) NP phenotypic marker- KRT19 (Scale bar= 100 μm). B, B’) Matrix catabolic enzyme- MMP13 (Scale bar= 100 μm). C-E"') Extracellular matrix molecules: C-C’’’) ARGxx, (D-D’’’) Collagen II, and (E-E’’’) Collagen X imaged at 10X and 20X (Left scale bars= 200 μm; Right scale bars= 50 μm). F-G’’’) pH regulatory enzymes: F-F"') LDHA and (G-G’’’) CA9 imaged at 10X and 20X (Left scale bars= 200 μm; Right scale bars= 50 μm). Dotted lines were drawn to demarcate different tissue compartments within the disc. All staining was performed using at least 3 animals/genotype corresponding to Grade 2.