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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Bone Miner Res. 2019 Dec 12;35(3):550–570. doi: 10.1002/jbmr.3908

Figure 5: Inhibition of H+/lactate efflux causes an increase in pyruvate metabolism and TCA cycle metabolites.

Figure 5:

A) PCA of metabolomic profiles from Ctrl and MCT-inhibited NP cells used for GC-MS analysis. B). Heatmap and hierarchical clustering of z-score normalized concentrations of u90 metabolites. C) Metabolites with significant concentration differences were identified using SAM with 5,000 permutations and 5% FDR threshold. D) Metaboanalyst Functional Annotation analysis of metabolites using KEGG pathway terms; p-value of each represented mouse metabolic pathway was calculated using hypergeometric test. E) Schematic of pyruvate metabolism via TCA cycle denoting fold-change of affected metabolites. (n= 5 independent experiments, 5 samples/condition).