FIGURE 2.

DNMT3L treatment restored the nuclear envelope-binding and histone core proteins that decreased during cellular aging. (A) Western blot analysis of extracts from young, old/presenescent and DNMT3L-treated MEFs using anti–lamin B1 and anti–α-tubulin antibodies. The distribution of the α-tubulin quantity was relatively stable among populations of young, old/presenescent and DNMT3L-treated MEFs with the same cell number. (B) Statistical results from repeated immunoblots assessing the relative quantity of lamin B1 normalized by α-tubulin. Mean ± SEM, n = 3. Asterisks indicate averages with significant differences at **p < 0.01, as determined by Student’s t-test. (C) Western blot analysis of extracts from the same set of cell lines using anti–H3 and anti–α-tubulin antibodies. (D) Statistical results from repeated immunoblots assessing the relative quantity of H3 normalized by α-tubulin. Mean ± SEM, n = 3. Asterisks indicate significant differences at **p < 0.01, Student’s t-test.