FIGURE 3.

Ectopic DNMT3L established a long-term effect on global H3K9me3 elevation and H3K9me3 enrichment on ERVs. (A) Western blot analysis of extracts from young, old/presenescent and DNMT3L-treated MEFs using anti–H3K9me3 antibody. Anti–H3 antibodies were used to indicate the relative expression on nucleosomes among samples. (B) ChIP-qPCR with anti-H3K9me3 antibody was used to assess the enrichment of H3K9me3 on representative Class I to Class III ERVs in the indicated cells. Mean ± SEM, n = 3. Rabbit IgG was used as a negative control.