FIGURE 2.

Typical cell partitioning approaches. (A) Individual cells and barcoded beads are separated by droplet encapsulation in oil using microfluidic devices. Following intra-droplet cellular lysis, cellular mRNAs are captured by the beads for downstream application. (B) Cells are allowed to sediment in wells. To ensure single-cell resolution, sedimentation either occurs at a dilution minimizing doublets or using microwells calibrated to allow deposition of only one cell. (C) Single cells are directly sorted in wells. The staining of surface markers provides the mean to enrich for the desired subset of cells.