Table 4.
PCR primers used for confirmation of bacterial infection.
| Bacterium | Primer sequence (5′-3′) |
|---|---|
| Porphyromonas gingivalis | F: AGT CGA GTT GCA GAC TCC GAT CC |
| R: AAC CCA CAT CGG TAG TTG CTA ACA G | |
| Fusobacterium nucleatum | F: AGA GTT TGA TCC TGG CTC AG |
| R: GTC ATC GTG CAC ACA GAA TTG CTG | |
| Tannerella forsythia | F: T CCC AAA GAC GCG GAT ATC A |
| R: ACG GTC GCG ATG TCA TTG T | |
| Treponema denticola | F: AGGGATATGGCAGCGTAGCA |
| R: TTGCGGGACTTAACCCAACA | |
| Universal (total bacteria) | F: TTGGGTTAAGTCCCGC |
| R: ATCCCCACCTTCCTCC |