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. 2020 Mar 10;10:4441. doi: 10.1038/s41598-020-61456-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR analysis of isolated proximal tubular epithelia from the mice that received cisplatin by FACS. (a) Isolation of tdTomato+ tubular epithelial cells using FACS as described in the experimental scheme in Fig. 3a. (b) qPCR of RNA from isolated tubular epithelia for the representative markers of mature tubules (lrp2), tubular injury (havcr1), dedifferentiation or mesenchymal markers (cd44 and vim), profibrotic cytokines (ccn2, tgfb1 and pdgfb), proinflammatory cytokines (tnf), and cell cycle (G1/S for pcna and fen1, G2/M for cdk1 and top2a). For all groups, data are means ± SEM, *p < 0.05 vs control, ^#p < 0.05 vs cisplatin.