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. 2020 Mar 10;10:4435. doi: 10.1038/s41598-020-61298-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The whole study flow. (A) Kaplan-Meier survival analysis and log-rank test were used to screen DFS-related mutant genes from TCGA database and AMC database. Then these genes were cross-verified in TCGA and AMC, and four DFS-related mutant genes were screened out in these two databases; (B) Boruta algorithm, Fisher’s test and Pearson’s test were used to screen race (Asian/non-Asian)-associated mutations from TCGA database; (C) Boruta algorithm, Fisher’s test and Pearson’s test were used to screen vascular invasion-associated mutations from TCGA, AMC and Inserm database; (D) The HCC data in TCGA were used to construct a model for predicting recurrence, and then AMC and 10 HCC patients in our center were used for verification.