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. 2020 Mar 10;11:1291. doi: 10.1038/s41467-020-15073-7

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

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Fig. 2 — a Lysates from HCT116 cells transfected with siRNAs to E6AP or hRpn10, as well as mock and scrambled control samples, were immunoprecipitated with Rpt3 antibodies. WCE and immunoprecipitates were immunoprobed as indicated. Cyclophilin B (Cyp B) is used as a loading control in a–c. b Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. c ΔRAZUL cells shown in b were transfected with full length or ΔRAZUL hRpn10 constructs and subjected to hRpt3 immunoprecipitation. Lysates or immunoprecipitates were probed with antibodies as indicated to the left of each panel. Source data are provided as a Source Data file.