Figure 5.

Total polyphenols are present in PEG-ENPs and exhibit antioxidant activity similar to ultra-ENPs, in vitro. (a) Total polyphenolics (TPC) were extracted from equal amount of ginger ENPs and assayed by Folin-Ciocalteu reagent as described in methods. Use of higher PEG6000 concentrations led to decreased enrichment of TPCs in ENPs. (b) Antioxidant activity of ultra and PEG-ENPs were measured using DPPH assay as described in methods. The antioxidant activity of TPCs encapsulated within ENPs were significantly reduced when 12% and 15% PEG were used, compared to 8%/10% PEG derived ENPs. (c) Antioxidant functionality of ENP associated bioactives on rescuing H₂O₂ induced cell death on RAW macrophages. Cell viability of RAW macrophages treated with H₂O₂ alone or in the presence of ENPs were measured by MTT assay (n = 3). ***P < 0.001, **P < 0.01.