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. 2020 Mar 10;10:4399. doi: 10.1038/s41598-020-60214-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mapping the inner plexiform layer (IPL). (A) Illustration of axial scans in the whole-mount retina of a transgenic mouse expressing tdTomato under the ChAT promotor and iGluSnFR via AAV transduction (Methods). (B) Axial x-z scan (256 × 160 pixels, 2 ms/line, zoom_XY,Z = 1.5, 0.75) with iGluSnFR expression (green) and ChAT bands (magenta). IPL borders and ChAT bands (solid and dashed lines, respectively) were defined manually (Methods). Note that the retina was flipped from (A), following the convention to show the photoreceptors pointing up. C, IPL border positions relative to ChAT bands (left; INL: 1.9 ± 0.1; GCL: −1.1 ± 0.1; n = 3/6/14 mouse/retinas/scans).