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. 2020 Mar 10;10:4399. doi: 10.1038/s41598-020-60214-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glutamate imaging in the inner plexiform layer. (A) Axial x-z scan (64 × 56 pixels, 1.6 ms/line) of the inner plexiform layer (IPL) in a whole-mount wild-type mouse retina expressing iGluSnFR ubiquitously after AAV-mediated transduction. (B) Correlation image (left) and distribution of correlation thresholds across the IPL (right). (C) ROIs extracted from scan in (A), pseudo-coloured by seed pixel correlation (for details on ROI extraction, see Methods). (D) Glutamate responses to local and global chirp stimulus for exemplary ROIs encircled in (C); Off (left) and On responses (right) are shown. (E) Distribution of all ROIs (black, n = 5,379) and ROIs that passed our quality threshold (grey, n = 3,893; Methods) recorded across the IPL (n = 6/8/37 mice/retinas/scans). (F) Number of ribbon synapses from different BC types per vertical IPL slice (Methods), estimated based on available EM data¹⁰.