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. 2020 Mar 10;10:4399. doi: 10.1038/s41598-020-60214-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Batch effects in axial x-z scans of the mouse inner plexiform layer (IPL). (A) Local chirp responses from ROIs located in the On sublamina of the IPL. From top: time course of chirp stimulus, heat map showing glutamate responses of ROIs from three scan fields (batches), average glutamate responses over ROIs in each batch, and magnified step and frequency responses. (B,C), Local chirp responses of ROIs in (A) projected onto their first two principal components (PCs), coloured by IPL depth (B) and batch (C).