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. 2020 Mar 10;10:4449. doi: 10.1038/s41598-020-61265-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Photomicrographs of histological samples in the anterior horn of the spinal cord of TDP-43 mice and wild-type controls. (a) Representative images of Nissl staining of motor neurons (black arrows), ChAT, Iba-1 and GFAP immunostained sections (b) Quantification of the different markers. Total average number of motor neurons (Nissl, ChAT+), microglia (Iba-1+) and astrocytes (GFAP+) is shown. Data are expressed as a percentage over the WT-Veh group. Values are expressed as means ± SEM, N ≥ 6 in each group. Data were assessed by one-way ANOVA followed by the Bonferroni test (*p < 0.05, **p < 0.01, ***p < 0.001 vs. WT-Veh group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. TDP-43-Veh). Scale bars = 200 μm.