Figure 4.

Effect of IGS-2.7 on TDP-43 phosphorylation in immortalized lymphocytes from control and sporadic ALS individuals. Immortalized lymphocytes were seeded at an initial density of 1 × 10⁶ × ml⁻¹ in absence or presence of IGS-2.7 (2.5 and 5 μM). 24 h after drugs addition, cells were harvested and processed for Western blotting analysis. (a) Densitometric measurements were performed on individual immunoblots and values indicate the mean of p-TDP-43 versus the corresponding total TDP-43 levels ± SEM for experiments carried out with 4 different cell lines for each group. (b) Representative immunoblot is shown. Data were assessed by one-way ANOVA and post hoc Fisher’s analysis (***p < 0.001 significantly different from control cells; ^##p < 0.01 and significantly different from untreated ALS cells).