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. 2020 Mar 10;10:4449. doi: 10.1038/s41598-020-61265-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression of CSNK1D and CSNK1E mRNA levels in post-mortem samples from control and ALS subjects and immunohistochemical analysis. (a) CSNK1D and CSNK1E mRNA levels in the anterior horn of the spinal cord and (b) frontal cortex area 8 of sALS cases and controls, *p < 0.05. (c) CK-1δ immunoreactivity in the anterior horn of the spinal cord at lumbar level in control and ALS cases showing CK-1δ immunoreactivity in the cytoplasm of neurons and neuronal processes in control. Similar reaction is found in the remaining motor neurons in devastated anterior horn in the ALS case together with increased CK-1δ immunoreactivity in reactive glial cells (arrows). Paraffin sections, slightly counterstained with haematoxylin; scale bar = 25 µm.