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. 2020 Mar 10;11:1297. doi: 10.1038/s41467-020-15061-x

Fig. 3. Effects of modified nucleosides, sites, and variants of MS2-binding motif and of variants of MS2CP on translational activation by CaVT.

Fig. 3

HeLa cells were co-transfected with hmAG1 mRNAs with MS2-binding motif (cap analog: A-cap, modified nucleosides: N1mΨ or Ψ/5mC), tagRFP mRNA, and an mRNA that expresses CaVT or its V29I mutant. The fluorescence was measured by a flow cytometer. a Schematic diagrams of hmAG1 mRNAs with MS2 binding motif. b CaVT (or its V29I mutant)-mediated fold change of the hmAG1/tagRFP ratio in cells transfected with the indicated reporter mRNAs. Means of the hmAG1/tagRFP ratio in each cell expressing both hmAG1 and tagRFP were calculated and normalized by the hmAG1/tagRFP ratio in reporter mRNA only samples. The bar graph shows the average of three independent experiments (mean ± SD). Source data are provided as a Source Data file. c Representative histograms of the hmAG1/tagRFP ratio in cells expressing both hmAG1 and tagRFP. While cells transfected with only reporter mRNAs are shown as red, cells transfected with mRNA that express CaVT or its V29I mutant are shown as cyan and orange, respectively.