Fig. 4. CaVT-mediated translational repression of the mRNA containing the strong binding motif.

a The predicted RNA secondary structure of MS2 binding motifs in the 5′ UTR of 2xScMS2(C)-hmAG1 mRNA. The structure was predicted by using ParasoR⁵⁴ and visualized by using VARNA⁵⁵. b CaVT (or its V29I mutant)-mediated fold change of the hmAG1/tagRFP ratio in cells transfected with the indicated reporter mRNAs. Means of the hmAG1/tagRFP ratio in each cell expressing both hmAG1 and tagRFP were calculated and normalized by the hmAG1/tagRFP ratio in reporter mRNA-only samples. The bar graph shows the average of three independent experiments (mean ± SD). Source data are provided as a Source Data file. c Representative histograms of the hmAG1/tagRFP ratio in cells expressing both hmAG1 and tagRFP. Cells transfected with only reporter mRNAs are shown as red, with mRNA that express CaVT as cyan, and with mRNA that express the CaVT V29I mutant as orange. d, e Representative superimposed dot plots of cells transfected with 2xScMS2(C)-hmAG1 mRNA (A-capped, 360 ng/well (d) or ARCA-capped, 20 ng/well (e)). Cells co-transfected with mRNA that express CaVT or its V29I mutant are shown as cyan, and cells transfected with only reporter mRNAs are shown as red.