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. 2020 Mar 10;10:4446. doi: 10.1038/s41598-020-61441-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of hUCBCs on the IL-8-mediated signalling pathway include the upregulation of Cxcl2 and CXCR2 expression, p38 activation, and subsequent degradation of IκB in the HI mouse brain. (a) The mRNA level of mouse Cxcl2, the IL-8 homologue was upregulated at 1 h post-hUCBC treatment according to RT-PCR analysis of the HI mouse brain (n = 5). (b) The upregulation of Cxcl2 expression after hUCBC administration in the HI mouse brain was consistent and significant according to real-time PCR assays 1 h post-hUCBC injection (n = 7). (c) The levels of mouse CXCR2 (the receptor for Cxcl2), p-p38, IκB, and ß-actin in the sham, HI, and hUCBC groups sacrificed on post-hUCBC days 1 and 3 were assayed using Western blotting to observe the consecutive changes after Cxcl2 upregulation. The level of CXCR2 was upregulated in parallel with p38 activation and the successive degradation of IκB after hUCBC administration. (d) The graph depicts the band intensity in p-p38 Western blotting. The data were obtained from triplicate results. Data are shown as the mean ± SEM (n = 5). Asterisks (*) indicate significant differences (*p < 0.05, **p < 0.01, one-way ANOVA) between groups. HI, hypoxic-ischaemic brain injury; hUCBCs, human umbilical cord blood mononuclear cells; UCB, HI mice subjected to hUCBC treatment.