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. 2020 Mar 10;10:4446. doi: 10.1038/s41598-020-61441-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo effects of p38 inhibition on the IL-8-mediated angiogenic pathway in the HI mouse brain. (a) Mice treated with the p38 inhibitor SB203580 (0.25 mg/kg) 30 min prior to hUCBC injection showed a decrease in the levels of p-MAPKAPK2 and IκB in response to hUCBC treatment, despite an increase in p-p38 after hUCBC injection according to Western blotting. (b) The graph depicts the band intensity of p-p38. (c) The protein levels of the angiogenic growth factors VEGF, bFGF, and PDGF and the endothelial marker CD31 were all downregulated following p38 inhibition according to Western blotting. (d–g) The graphs depict the band intensity in each Western blot assay. Data are shown as the mean ± SEM (each n ≥ 3). The data shown in the graphs were obtained from triplicate results. Asterisks (*) indicate significant differences (*p < 0.05, one-way ANOVA). HI, hypoxic-ischaemic brain injury; hUCBCs, human umbilical cord blood mononuclear cells; UCB, HI mice subjected to hUCBC treatment; UCB+SB, mice treated with SB203580 prior to hUCBC treatment.