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. 2020 Mar 10;10:4397. doi: 10.1038/s41598-020-61022-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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viSNE profile of sm14⁺16⁺ monocytes. Small and large monocyte populations identified with SPADE (Supplementary Fig. S5) were analysed with viSNE and specific expression profiles of adhesion molecules were identified in donors. The expression of CD43, CD49d and CD62L (x, y, z) in sm14⁺16⁺ monocytes was represented here in dot plot profiles, with CD62L expression shown according to a colour scale (right hand side axis). Profiles shown in (a–e) correspond to monocyte sub-populations a, b, c, d, e identified in Table 2. In panel a, a schematic representation of monocyte populations identified in profile a and that are found in various combinations in profiles b, c, d, e is shown. Plots shown are from representative donors expressing a to e profiles.