Figure 5.

BMP2 promoted the expansion of MDSCs in vitro. (A) Representative flow cytometric images and quantitative analyses of monocyte-derived MDSCs and polymorphonuclear-derived MDSCs. Cells from bone marrow of ICR mice were stimulated with rhBMP2 or rhBMP2 with LDN-193189 in vitro and then stained with indicated antibodies and subjected to flow cytometric analyses. *P < 0.05, **P < 0.01. (B) Representative flow cytometric images and quantitative analyses of monocyte-derived MDSCs and polymorphonuclear-derived MDSCs. Cells from bone marrow of C57BL/6 mice were stimulated with rhBMP2 or rhBMP2 with LDN-193189 in vitro, and then stained with indicated antibodies and subjected to flow cytometric analyses. *P < 0.05, **P < 0.01. (C) Phosphorylation levels of Smad1/5, Stat3, and Erk after the bone marrow of ICR and C57BL/6 mouse were treated with BMP2. Cell lysates were harvested to be subjected to Western blot. β-Actin was the reference for all the blots. Total Smad1/5, Stat3, and Erk were the references for the phosphorylated proteins. (D) Comparison of relative iNOS, Arg1, and IL6 mRNA levels of bone marrow cells treated with BMP2. *P < 0.05.