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. 2020 Mar 4;10:194. doi: 10.3389/fonc.2020.00194

Figure 6.

Figure 6

BMP2 activated MDSCs to enhance liver cancer growth via IL6. (A) About 3000 Hepa1-6 cells were cultured in each well of 96-well plates with control conditional medium or MDSC secreting conditional medium. Relative cell intensity was measured with a cell-counting kit after indicated time. **P < 0.01. (B) About 3000 H22 cells were cultured in each well of 96 well plates with control conditional medium or MDSC secreting conditional medium. Relative cell intensity was measured with a cell-counting kit after indicated time. **P < 0.01. (C) IL6 concentration from control conditional medium or MDSC secreting conditional medium were compared. **P < 0.01. (D) IL6 concentration in peripheral blood and tumor tissues of rhBMP2 solution intravenously injected ICR mice for NS and rhBMP2 groups were compared. *P < 0.05, **P < 0.01. (E) IL6 concentration in peripheral blood and tumor tissues of rhBMP2 collagen gels implanted ICR mice for NS and rhBMP2 groups were compared. *P < 0.05. (F) About 3000 Hepa1-6 cells were cultured in each well of 96-well plates with vehicle or IL6. Relative cell intensity was measured with a cell-counting kit after indicated time. *P < 0.05, **P < 0.01. (G) About 3000 Hepa1-6 cells were cultured in each well of 96-well plates with control conditional medium or MDSC secreting conditional medium. Control IgG or the anti-IL6 antibody were treated. Relative cell intensity was measured with a cell-counting kit after indicated time. *P < 0.05, **P < 0.01. (H) Immunohistochemistry images of ki67 subcutaneous H22 or Hepa1-6 tumor samples from rhBMP2 collagen gel-implanted ICR mice. Scale bar: 50 μm. Average percentages of Ki67-positive cells of at least three fields were shown below. **P < 0.01. (I) Pearson correlation of BMP2 and ARG1 expression were analyzed in human HCC. Data obtained from the TCGA Research Network. (J) Pearson correlation of BMP2 and IL6 expression were analyzed in human HCC. Data obtained from the TCGA Research Network. (K) Schematic diagram revealed the effects of BMP2 on the growth of liver cancer.