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. 2020 Mar 4;11:204. doi: 10.3389/fphar.2020.00204

FIGURE 2.

FIGURE 2

PUR alleviates HI-induced apoptosis at 72 h post-HI insult. (A) Levels of Bax and Bcl-2 within the ipsilateral cortex were measured with use of western blotting. Quantification of protein levels of Bax and Bcl-2 were determined with use of Image-Pro Plus 6⋅0 (N = 4/group). (B) Double immunofluorescent staining of active caspase-3 (red) and NeuN (green) within the ipsilateral cortex. Scale bar = 50 μm. (C) Quantification of active caspase-3/NeuN-positive cells (N = 4/group). Six images (20×) were captured randomly for each section per animal. (D) Immunoblotting analysis of active caspase-3, caspase-3 within the ipsilateral cortex. (E) Image-Pro Plus 6.0 was used to quantify protein levels of caspase-3 and active caspase-3. Results are expressed as the ratio of active caspase-3/caspase-3 (N = 4/group). Values represent the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001 according to ANOVA with Bonferroni correction.