TABLE 1.
All reagents used in this study.
| Primary antibodies | Dilution | Cat. No. | Producer |
| 1.1 Antibodies used for Western blot analysis | |||
| Anti-β-actin | 1:1000 | TA-09 | Zhongshan Golden Bridge Biotechnology, Beijing, China |
| Anti-Bax | 1:1000 | 50599-2-Ig | Proteintech Group, United States |
| Anti-Bcl-2 | 1:1000 | 12789-1-AP | Proteintech Group, United States |
| Anti-Cleaved caspase-3 | 1:500 | Asp175, #9661 | Cell Signaling, Beverly, MA, United States |
| Anti-Caspase-3 | 1:1000 | 19677-1-AP | Proteintech Group, United States |
| Anti-Nrf2 | 1:500 | 16396-1-AP | Proteintech Group, United States |
| Anti-Syn | 1:1000 | D35E4, #5461 | Cell Signaling, Beverly, MA, United States |
| Anti-PSD95 | 1:1000 | D74D3, #3409 | Cell Signaling, Beverly, MA, United States |
| Anti-Shh | 1:1000 | 20697-1-AP | Proteintech Group, United States |
| Anti-Ptch | 1:500 | ab53715 | Abcam, Cambridge, United Kingdom |
| Anti-Gli-1 | 1:500 | ab151796 | Abcam, Cambridge, United Kingdom |
| 1.2 Antibodies used for immunofluorescence and immunohistochemistry staining | |||
| Anti-NeuN | 1:100 | ab104224 | Abcam, Cambridge, United Kingdom |
| Anti-Syn (mouse) | 1:100 | 60191-1-Ig | Proteintech Group, United States |
| Anti-PSD95 (rabbit) | 1:100 | 20665-1-AP | Proteintech Group, United States |
| Anti-Cleaved caspase-3 | 1:100 | Asp175, #9661 | Cell Signaling, Beverly, MA, United States |
| Anti-Iba-1 | 1:100 | GTX632426 | GeneTex, Inc.,Irvine, CA, United States |
| 1.3 Other major reagents used in this study | |||
| Purmorphamine | Selleck Chemicals (Houston, TX, United States) | ||
| Cyclopamine | Selleck Chemicals (Houston, TX, United States) | ||
| Radioimmunoprecipitation assay (RIPA) | Beyotime Institute of Biotechnology, Jiangsu, China | ||
| Phenylmethane-sulfonyl fluoride (PMSF) | Beyotime Institute of Biotechnology, Jiangsu, China | ||
| PhosSTOP phosphatase inhibitor | Roche Diagnostics Gmbh, Indianapolis, IN, United States | ||
| BCA protein assay kit | CWBIO, Haimen, Jiangsu, China | ||
| 5 × loading buffer | Beyotime Institute of Biotechnology, Jiangsu, China | ||
| PVDF membranes | Millipore, United States | ||
| Enhanced chemiluminescence | Millipore, United States | ||
| TRIzol reagent | CWBIO, Haimen, Jiangsu, China | ||
| RevertAid First Strand cDNA Synthesis Kit | Thermo scientific, Waltham, MA, United States | ||
| GolView | Beijing Solarbio Science and Technology Co, China | ||
| 4,6-Diamidino-2-phenylindole (DAPI) | Beyotime Institute of Biotechnology, Jiangsu, China | ||
| Dihydroethiduim | Beyotime Institute of Biotechnology, Jiangsu, China | ||
| Terminal deoxynucleotidyl transferase dUTP-mediated nick end labeling (TUNEL) Apoptosis Detection kit | KeyGen Bio Tech Co. (Nanjing, China). | ||
| Rhodamine (TRITC)-conjugated goat anti-rabbit IgG | Proteintech Group, United States | ||
| Fluorescein (FITC)-conjugated affinipure goat anti-mouse IgG | Proteintech Group, United States | ||
| Peroxidase-conjugated goat anti-rabbit/mouse IgG | Zhongshan Golden Bridge Biotechnology | ||
| 2,3,5-Triphenyltetrazolium chloride monohydrate (TTC) | Sigma–Aldrich, St. Louis, MO, United States | ||
| Cresyl violet acetate | Sigma–Aldrich, St. Louis, MO, United States | ||
| APC anti-mouse CD45 | Biolegend (103112) | ||
| FITC anti-mouse/human CD11b | Biolegend (101206) | ||