Figure 1.

In vivo biodistribution of radiolabelled [¹¹¹In]L-ALD in A375Pβ6.luc tumours, after single dose administration via tail vein injection in NSG mice. (A) Bioluminescence imaging of three A375Pβ6 tumour models in NSG mice. (B) Blood clearance profile of [¹¹¹In]L-ALD expressed as % ID. (C) Organ biodistribution of [¹¹¹In]L-ALD expressed as per cent injection dose per gram of organ (%ID/g). (D) Uptake of [¹¹¹In]L-ALD in SC and IP tumour models at 24 h expressed as %ID/g. (E) Excretion profile of [¹¹¹In]L-ALD expressed as % ID. Subcutaneous (SC), intraperitoneal (IP) and pseudo-metastatic lung tumours were established by inoculating NSG mice by i.v. injection of 5 x 10⁵ A375Pβ6 cells, i.p. injection of 5 x 10⁵ A375Pβ6 cells and s.c. injection of 5 x 10⁶ A375Pβ6 cells, respectively. A375Pβ6 cells were transduced with firefly luciferase and tumour growth was monitored by bioluminescence imaging (IVIS Lumina series III In Vivo Imaging system, Perkin-Elmer). Tumours were established for three weeks prior to use. Mice were i.v. injected with [¹¹¹In]L-ALD at a dose of 2 μmol lipid/mouse. Blood samples (5 µl) were taken at 5, 10 and 30 min and 1, 4 and 24 h. Urine and faeces were collected. After 24 h the mice were sacrificed, and liposome uptake was quantified by gamma counting. Data was expressed as mean ± SD (n=6-10 mice per group). ** p < 0.01