Figure 2.

In vivo whole body 3D SPECT/CT imaging of [¹¹¹In]tropolone and [¹¹¹In]Vγ9Vδ2 T cells in SC-tumour-bearing SCID/Beige mice. γδ T cells were radiolabelled by incubation with [¹¹¹In]tropolone. Any [¹¹¹In]tropolone not incorporated into the γδ T cells was removed by centrifugation. Tumour bearing mice were intravenously injected with 5 x 10⁶ [¹¹¹In]γδ T cells or the equivalent amount of [¹¹¹In]tropolone. The mice were imaged immediately and after 4 and 24 h. The γδ T cells go directly to the lung and then redistribute to the liver, spleen and kidneys over time.​ The pattern of biodistribution for [¹¹¹In]tropolone was markedly different to that of the [¹¹¹In]γδ T cells, suggesting that the cells were stably labelled and have been successfully purified from any free [¹¹¹In]tropolone.​