Figure 3.

In vivo biodistribution of radiolabelled γδ T cells in A375Pβ6.luc tumour bearing NSG mice, after single dose administration via tail vein injection. NSG mice were inoculated with luciferase-expressing A375Pβ6.luc tumour cells to form (A) subcutaneous (SC) (B) intraperitoneal (IP) or (C) experimental metastatic lung tumours. Tumours were allowed to establish for three weeks prior to the biodistribution study. Mice were i.v. injected with [¹¹¹In]γδ T cells at a dose of 5 x 10⁶ γδ T cells/mouse. Mice were pre-treated with 0.5 μmol ALD or L-ALD, 24 h prior to injection of γδ T cells. Blood samples (5 µl) were taken at 0.5, 1, 4 and 24 h. After 24 h the mice were sacrificed and the biodistribution of γδ T cells was quantified by gamma counting. Results are expressed as percent injection dose per gram of organ (%ID/g organ) or % ID/blood for blood samples. Data are expressed as mean ± SD (n=4 mice per group). Statistical significance was indicated in comparison to PBS-injected mice. *p < 0.05, (one-way ANOVA).