Figure 4.

In vivo tumour uptake of radiolabelled [¹¹¹In]γδ T cells in A375Pβ6.luc tumours, after single dose administration via tail vein injection in NSG mice. NSG mice were inoculated with the A375Pβ6.luc cell line to form subcutaneous (SC), intraperitoneal (IP) or experimental metastatic lung tumours. Mice were i.v. injected with 0.5 μmol ALD or L-ALD 24 h prior to i.v. injection of [¹¹¹In]γδ T cells at a dose of 5 x 10⁶ γδ T cells/mouse. (A) Tumour uptake of [¹¹¹In]γδ T cells expressed as % ID/g of tumour in SC and IP tumour-bearing mice. (B) Lung uptake of [¹¹¹In]γδ T cells expressed as % ID/g of lung in SC, IP and experimental metastatic lung tumour-bearing mice. Pre-injection of ALD or L-ALD did not significantly alter the tumour uptake of γδ T cells. Data are expressed as mean ± SD (n=4 mice per group). Statistical analysis was down by Kruskal-Wallis test followed by Dunn's test to compare the differences between individual group (* p < 0.05).