FIG 3.

Functional validation of sphingosine-1-phosphate receptor 1 in mice infected with P. aeruginosa. C57BL/6NCrl mice were infected with 5 × 10⁶ CFU of the P. aeruginosa AA2 clinical strain or challenged with PBS as a control. Next, mice were treated with the Ex26 compound at a dose of 3 mg/kg (n = 14) or with the 3% DMSO vehicle only (n = 12). (A) Survival rates of mice infected with P. aeruginosa and treated with the Ex26 compound or the vehicle or challenged with PBS and treated with the Ex26 compound (*, P < 0.05 by a Mantel-Cox test). (B) White blood cell (WBC) count in mice (n = 3) at T₀ (before infection), T₁ (6 h), and T₂ (24 h) after infection with P. aeruginosa and Ex26 treatment.