TABLE 1.
Strain(s) | MIC (mg/liter) ofa
: |
|||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
TIG (+NMP) | ERV | DOX | TET | MIN | OXY | CTC | CTX | CAZ | CQM | FEP | CIP | STR | GEN | |
Pandrug-resistant K. pneumoniae
strains |
||||||||||||||
AH8I, AH6I, AH25I, AH28I, and AH33Ib | 16 (<0.0156) | 32 | 64 | >128 | 64 | >128 | >128 | >128 | >128 | >128 | >32 | >64 | >128 | >128 |
E. coli strains | ||||||||||||||
J53 | 0.5 | 0.5 | 4 | 1 | 4 | 8 | 4 | 0.03 | 0.25 | 0.03 | 0.03 | 0.008 | 1 | 0.125 |
J53/pHNAH8I-1 | 4 | 4 | 16 | 8 | 16 | 16 | 16 | 0.06 | 0.25 | 0.5 | 0.25 | 0.03 | 8 | 0.25 |
DH5α | 0.25 | 0.5 | 2 | 1 | 2 | 2 | 4 | 0.03 | 0.125 | 0.03 | 0.03 | 0.002 | 1 | 0.125 |
DH5α/pHNAH8I-1 | 8 | 16 | 32 | 32 | 32 | 64 | 32 | 0.5 | 1 | 2 | 0.25 | 0.03 | 128 | 4 |
DH5α/pHSG575 | 0.25 | 0.5 | 2 | 1 | 2 | 4 | 4 | 0.03 | 0.125 | 0.03 | 0.03 | 0.002 | 1 | 0.125 |
DH5α/pHSG575-tmexCD1 | 1 | 2 | 4 | 4 | 4 | 8 | 16 | 0.125 | 0.25 | 0.25 | 0.25 | 0.0156 | 4 | 0.25 |
DH5α/pHSG575-tnfxB1-tmexCD1 | 1 | 2 | 4 | 4 | 4 | 8 | 16 | 0.125 | 0.25 | 0.25 | 0.25 | 0.0156 | 4 | 0.25 |
DH5α/pHSG575-tmexCD1-toprJ1 | 4 | 8 | 32 | 16 | 16 | 16 | 32 | 0.25 | 1 | 1 | 0.5 | 0.03 | 16 | 1 |
DH5α/pHSG575-tnfxB1-tmexCD1- toprJ1 | 4 | 8 | 32 | 16 | 16 | 16 | 32 | 0.25 | 1 | 1 | 0.5 | 0.03 | 16 | 1 |
K. pneumoniae strains | ||||||||||||||
YX94 | 0.5 | 1 | 2 | 2 | 4 | 4 | 8 | 0.03 | 0.06 | 0.06 | 0.06 | 0.008 | 8 | 0.25 |
YX94/pHNAH8I-1 | 8 | 16 | 16 | 8 | 16 | 16 | 16 | 0.125 | 0.5 | 2 | 1 | 0.25 | >128 | 1 |
YX94/pHSG575 | 0.5 | 1 | 2 | 2 | 4 | 8 | 4 | 0.03 | 0.06 | 0.06 | 0.06 | 0.008 | 8 | 0.25 |
YX94/pHSG575-tnfxB1-tmexCD1- toprJ1 | 16 | 16 | 32 | 32 | 32 | 32 | 16 | 0.125 | 0.5 | 2 | 0.5 | 0.25 | >128 | 4 |
AH58I | 0.5 | 1 | 4 | 8 | 4 | 8 | 4 | >128 | >128 | >128 | >32 | 32 | 16 | 4 |
AH58I/pHNAH8I-1 | 8 | 16 | 32 | 32 | 16 | 32 | 16 | >128 | >128 | >128 | >32 | 64 | >128 | 16 |
S. Typhimurium strains | ||||||||||||||
HN227/pHSG575 | 0.5 | 1 | 4 | 2 | 4 | 8 | 4 | 0.125 | 0.5 | 0.125 | 0.06 | 0.008 | 8 | 0.25 |
HN227/pHSG575-tnfxB1- tmexCD1-toprJ1 | 16 | 16 | 32 | 32 | 32 | 128 | 32 | 0.5 | 2 | 8 | 4 | 0.25 | 128 | 4 |
TIG, tigecycline; ERV, eravacycline; DOX, doxycycline; TET, tetracycline; MIN, minocycline; OXY, oxytetracycline; CTC, chlortetracycline; CTX, cefotaxime; CAZ, ceftazidime; CQM, cefquinome; FEP, cefepime; CIP, ciprofloxacin; STR, streptomycin; GEN, gentamicin. MICs of tigecycline were determined by microdilution and those of other antibiotics by agar dilution. NMP was used with all the tested antibiotics, but the results are not shown.
The imipenem MICs against the five K. pneumoniae isolates were all 8 mg/liter, and MICs of fosfomycin were all 32 mg/liter.