TABLE 1.
MICs against parental strains, transformants, and transconjugants
| Strain(s) | MIC (mg/liter) ofa
: |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TIG (+NMP) | ERV | DOX | TET | MIN | OXY | CTC | CTX | CAZ | CQM | FEP | CIP | STR | GEN | |
| Pandrug-resistant K. pneumoniae
strains |
||||||||||||||
| AH8I, AH6I, AH25I, AH28I, and AH33Ib | 16 (<0.0156) | 32 | 64 | >128 | 64 | >128 | >128 | >128 | >128 | >128 | >32 | >64 | >128 | >128 |
| E. coli strains | ||||||||||||||
| J53 | 0.5 | 0.5 | 4 | 1 | 4 | 8 | 4 | 0.03 | 0.25 | 0.03 | 0.03 | 0.008 | 1 | 0.125 |
| J53/pHNAH8I-1 | 4 | 4 | 16 | 8 | 16 | 16 | 16 | 0.06 | 0.25 | 0.5 | 0.25 | 0.03 | 8 | 0.25 |
| DH5α | 0.25 | 0.5 | 2 | 1 | 2 | 2 | 4 | 0.03 | 0.125 | 0.03 | 0.03 | 0.002 | 1 | 0.125 |
| DH5α/pHNAH8I-1 | 8 | 16 | 32 | 32 | 32 | 64 | 32 | 0.5 | 1 | 2 | 0.25 | 0.03 | 128 | 4 |
| DH5α/pHSG575 | 0.25 | 0.5 | 2 | 1 | 2 | 4 | 4 | 0.03 | 0.125 | 0.03 | 0.03 | 0.002 | 1 | 0.125 |
| DH5α/pHSG575-tmexCD1 | 1 | 2 | 4 | 4 | 4 | 8 | 16 | 0.125 | 0.25 | 0.25 | 0.25 | 0.0156 | 4 | 0.25 |
| DH5α/pHSG575-tnfxB1-tmexCD1 | 1 | 2 | 4 | 4 | 4 | 8 | 16 | 0.125 | 0.25 | 0.25 | 0.25 | 0.0156 | 4 | 0.25 |
| DH5α/pHSG575-tmexCD1-toprJ1 | 4 | 8 | 32 | 16 | 16 | 16 | 32 | 0.25 | 1 | 1 | 0.5 | 0.03 | 16 | 1 |
| DH5α/pHSG575-tnfxB1-tmexCD1- toprJ1 | 4 | 8 | 32 | 16 | 16 | 16 | 32 | 0.25 | 1 | 1 | 0.5 | 0.03 | 16 | 1 |
| K. pneumoniae strains | ||||||||||||||
| YX94 | 0.5 | 1 | 2 | 2 | 4 | 4 | 8 | 0.03 | 0.06 | 0.06 | 0.06 | 0.008 | 8 | 0.25 |
| YX94/pHNAH8I-1 | 8 | 16 | 16 | 8 | 16 | 16 | 16 | 0.125 | 0.5 | 2 | 1 | 0.25 | >128 | 1 |
| YX94/pHSG575 | 0.5 | 1 | 2 | 2 | 4 | 8 | 4 | 0.03 | 0.06 | 0.06 | 0.06 | 0.008 | 8 | 0.25 |
| YX94/pHSG575-tnfxB1-tmexCD1- toprJ1 | 16 | 16 | 32 | 32 | 32 | 32 | 16 | 0.125 | 0.5 | 2 | 0.5 | 0.25 | >128 | 4 |
| AH58I | 0.5 | 1 | 4 | 8 | 4 | 8 | 4 | >128 | >128 | >128 | >32 | 32 | 16 | 4 |
| AH58I/pHNAH8I-1 | 8 | 16 | 32 | 32 | 16 | 32 | 16 | >128 | >128 | >128 | >32 | 64 | >128 | 16 |
| S. Typhimurium strains | ||||||||||||||
| HN227/pHSG575 | 0.5 | 1 | 4 | 2 | 4 | 8 | 4 | 0.125 | 0.5 | 0.125 | 0.06 | 0.008 | 8 | 0.25 |
| HN227/pHSG575-tnfxB1- tmexCD1-toprJ1 | 16 | 16 | 32 | 32 | 32 | 128 | 32 | 0.5 | 2 | 8 | 4 | 0.25 | 128 | 4 |
a
TIG, tigecycline; ERV, eravacycline; DOX, doxycycline; TET, tetracycline; MIN, minocycline; OXY, oxytetracycline; CTC, chlortetracycline; CTX, cefotaxime; CAZ, ceftazidime; CQM, cefquinome; FEP, cefepime; CIP, ciprofloxacin; STR, streptomycin; GEN, gentamicin. MICs of tigecycline were determined by microdilution and those of other antibiotics by agar dilution. NMP was used with all the tested antibiotics, but the results are not shown.
b
The imipenem MICs against the five K. pneumoniae isolates were all 8 mg/liter, and MICs of fosfomycin were all 32 mg/liter.