TABLE 2.

Surface plasmon resonance analysis of carbohydrates similar to the pilin disaccharide^a

^aTo determine the galactose specificity of the interaction between the I-domain and the gonococcal pilin-linked disaccharide, SPR was performed using carbohydrates with terminal α- or β-galactose in various linkages. Results shown are the means ± standard errors from three replicate experiments (N/T; not tested). These data showed that the human I-domain can form a high-affinity interaction with various terminal galactose structures. The G2 peptide is a biotin-Ahx-labeled peptide (Ahx is a six-carbon spacer). Sucrose was used as a negative control and showed no concentration-dependent interaction (NCDI; no interaction at any concentration between 0.0078 μM and 2 μM).