FIG 7.

Quantitative PCR confirms altered expression of bacterial quorum sensing and T7SS genes during VPE25 infection. mRNA transcript levels of quorum sensing regulon genes, including fsr regulatory genes (A), fsr-induced genes (B), and fsr-repressed genes (C) are differentially expressed during VPE25 infection. (D) Progression of the lytic cycle induces the expression of T7SS genes. Expression is the fold change relative to untreated samples at the same time points. Data represent the average of three replicates ± the standard deviation. *P < 0.01 to 0.0001 and **P < 0.00001 by unpaired Student’s t test.