TABLE 1.

Determined diffusion coefficients and dwell times of DgcK-mVenus in different B. subtilis NCIB 3610 strains^a

Strain with DgcK-mVenus	Static D ± SD (μm² sˉ¹)	Mobile D ± SD (μm² sˉ¹)	Static fraction ± SD (%)	Mobile fraction ± SD (%)	Dwell time (s)
NCIB 3610b	0.079 ± 0.001	0.657 ± 0.081	69.7 ± 2.1	30.3 ± 2.1	0.049 ± 0.002
ΔydaK strain	0.079 ± 0.001	0.657 ± 0.081	56.7 ± 4.7	43.3 ± 4.7	0.041 ± 0.002
Stressed with 4% EtOH	0.079 ± 0.001	0.657 ± 0.081	79.0 ± 4.4	21.0 ± 4.4	0.058 ± 0.006
Pxyl-ydaKLMN strain	0.079 ± 0.001	0.657 ± 0.081	90.7 ± 4.5	9.3 ± 4.5	0.067 ± 0.001
NCIB 3610c	0.075 ± 0.004	0.383 ± 0.040	70.3 ± 10.0	29.7 ± 10.0	0.070 ± 0.002
ΔdgrA strain	0.075 ± 0.004	0.383 ± 0.040	18.3 ± 1.5	81.7 ± 1.5	0.035 ± 0.002

^aD, diffusion coefficient; dwell time, time period in which a track stays within a radius of 230 nm (2.3 pixels). The errors were calculated from the results of three biological replicates.

^bWild type for ydaK experiments.

^cWild type for dgrA experiments.